Kansas State Veterinary Diagnostic Laboratory

Molecular Diagnostics

molecular diagnostics banner

The Molecular Diagnostics laboratory of the KSVDL provides high quality diagnostic services to practicing veterinarians, the KSU-Veterinary Health Center, researchers and industry. Traditional PCR testing is augmented with molecular genotyping and DNA sequencing services to assist clinicians in identifying infectious agents to the genus, species and strain levels. Testing services include identification of a broad range of viral, bacterial and vector borne pathogens.

Tests

  • RAPD-PCR strain analysis of Actinobacillus pleuropneumoniae Bordetella, Moraxella bovis/ ovis
  • Real-Time PCR testing for Canine Ehrlichiosis/Anaplasmosis and West Nile Virus
  • DNA sequencing and sequence analysis of BVDV, PCV2, and PRRSV
  • PCR based identification of Actinobacillus pleuropneumoniae, Bordetella, Haemophilus parasuis, Streptococcus equi subsp.equi, Streptococcus zooepidemicus, Streptococcus suis,Streptococcus canis, Streptococcus bovis, Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis
  • DNA sequencing and sequence analysis of BVDV, PCV2, and PRRSV
  • E. coli and Clostridium perfringes genotyping
  • Tritrichomonas foetus for Bulls

Available Strain Analysis

  • RAPD-PCR strain analysis of Actinobacillus pleuropneumoniae Bordetella, Moraxella bovis/ ovis
  • Serotype identification (1-10)
  • PCR Based Strain Analysis (RAPD)
  • PCR based identification of Actinobacillus pleuropneumoniae, Bordetella, Haemophilus parasuis, Streptococcus equi subsp.equi, Streptococcus zooepidemicus, Streptococcus suis,Streptococcus canis, Streptococcus bovis, Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis
  • Actinobacillus pleuropneumoniae (Genotyping serotypes 1-15)
  • Bordetella bronchiseptica(discriminate field and vaccine strain)
  • Moraxella bovis(discriminate M.bovis from M.ovis) PCR Based Identification
  • Actinobacillus pleuropneumoniae
  • Bordetella bronchiseptica
  • Bordetella avium
  • Hemophilus parasuis
  • Streptococcus equi subsp.equi
  • Streptococcus species (Multiplex for unknowns or species specific)
  • S.bovis, S.canis, S.suis, S.porcinus, S.agalactiae, S.dysgalactiae and S.uberis

Diagnostic Services (Tested by Real Time RT-PCR Pathogens)

  • Ehrlichia canis
  • Ehrlichia chaffeensis
  • Ehrlichia ewingii
  • Anaplasma platys
  • Anaplasma phagocytophilum

West Nile Virus Samples Accepted (Tested by Real Time RT-PCR Pathogens)

  • Equine Brain Tissues
  • Oral / Cloacal Swabs of Birds
  • Mosquito Pools

Tritchomonas Foetus

Kansas State Veterinary Diagnostic Laboratory expands Tritrichomonas foetus diagnostic capability to include pooling, and alternative collection media.
KSVDL has added the real-time PCR assay of McMillen and Lew (Veterinary Parasitology 141 (2006) 204-215) in the diagnosis of Tritrichomonas foetus. Samples for these tests should be submitted in the InPouch™ TF Pouch or InPouch™ TF Tube (one pouch or tube/bull).
Preputial samples should be immediately inoculated in the InPouch™ TF Pouch or Tube system and shipped to be received by the lab within 72 hours after collection. As KSVDL is open Saturday a.m., samples shipped on Friday for Saturday delivery are acceptable.
Pouches may be ordered in bulk from BioMed Diagnostics (1-800-964-6466) or by the pouch from KSVDL (1-866-512-5650).

Trichomoniasis Infections in Beef Herds

Proper Tritrichomonas foetus sampling techniques and appropriate sample handling:

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If you have questions about a specific test conducted by this lab, please contact Client Services at (785) 532-5650, toll free at 1-866-512-5650 or clientcare@vet.k-state.edu for special testing submission and fees.