July 2019

New Tests at KSVDL

Bovine Pinkeye (IBK) PCR Panel (MDL:3330)

This test is specific for:

Bacterial pathogens:	Viral pathogen:
Moraxella bovoculi	Bovine herpes virus type 1 (IBR)
Moraxella bovis
Mycoplasma bovoculi
Mycoplasma bovis

Sample: eye swabs submitted in a sterile container with a few drops (0.5 ml) of saline or PBS.

Test schedule: Monday through Friday

Expected turnaround time: 1-2 days.

The results are reported out as Positive, Negative, Suspect, with Ct values.

Blue-green Algae (TOX-1003)

Microscopic examination of water

Sample: 500 ml water that includes pond scum

Test schedule: Monday through Friday

Expected turnaround time: 1 day

Microcystin (TOX-1072)

Test is specific for a toxin produced by some blue-green algae

Sample:

1. 500 ml water that includes pond scum
2. One pound of rumen contents

Test schedule: Monday through Friday

Expected turnaround time: 1 day

Molecular Serotyping of Salmonella Isolates (MDL-1027)

Species: All

Sample:

1. culture plates, slants, purified DNA
2. loop of culture inoculated into 2.0 ml of enrichment media
3. minimum of 15 ul DNA at concentration of 10 ng/ul or higher

Test schedule: Monday through Friday

Expected turnaround time: 3-4 days.

New Immunology Tests

• Anti-nuclear Antibody
• Rheumatoid Factor
• Immuno CSF
• Immuno Peritoneal
• Immuno Pleural
• Immunophenotyping of Bone Marrow
• Immunophenotyping of Lymph Node Aspirate
• Immunophenotyping of Peripheral Blood
• Immunofluorescent Antibody Assay

NEW Swine Diarrhea Panels!!

Swine Diarrhea PCR Full Panel (MDL-6020)

• Enterotoxigenic Escherichia coliF6, F18, F41, K88, K99, st1a, st1b, elt, and stx2e
• Clostridium perfringensAlpha & Beta toxins
• Clostridium difficiletoxins A & B
• Porcine epidemic diarrhea virus (PEDV)
• Porcine deltacoronavirus (PDCov)
• Transmissible gastroenteritis virus (TGEV)
• Salmonella spp.
• Lawsonia intracellularis
• generic Escherichia coli
• Brachyspira hyodysenteriae
• Brachyspira pilosicoli
• Brachyspira hampsonii
• Porcine rotavirus A, B and C

Swine Growing Pig Diarrhea PCR Panel (MDL-6023)

• Enterotoxigenic Escherichia coliF6, F18, F41, K88, K99, st1a, st1b, elt, and stx2e
• Salmonella spp.
• Lawsonia intracellularis
• generic Escherichia coli
• Brachyspira hyodysenteriae
• Brachyspira pilosicoli
• Brachyspira hampsonii

Swine Suckling Pig Diarrhea PCR Panel (MDL-6021)

• Enterotoxigenic Escherichia coliF6, F18, F41, K88, K99, st1a, st1b, elt, and stx2e
• Clostridium perfringensAlpha & Beta toxins
• Clostridium difficiletoxins A & B
• Porcine epidemic diarrhea virus (PEDV)
• Porcine deltacoronavirus (PDCov)
• Transmissible gastroenteritis virus (TGEV)
• Porcine rotavirus A, B and C

Swine Weaned Pig Diarrhea PCR Panel (MDL-6022)

• Enterotoxigenic Escherichia coliF6, F18, F41, K88, K99, st1a, st1b, elt, and stx2e
• Porcine epidemic diarrhea virus (PEDV)
• Porcine deltacoronavirus (PDCov)
• Transmissible gastroenteritis virus (TGEV)
• Salmonella spp.
• Lawsonia intracellularis
• generic Escherichia coli
• Porcine rotavirus A, B and C

For all Swine Diarrhea Panels:

Samples

• (5 gm) Feces or Intestinal content
• (5 gm) Intestine
• Fecal swabs
• A gel bacterial swab is not acceptable

Test schedule: Monday through Friday

Expected turnaround time: 2-3 days for Enterotoxigenic E. coli

1-2 days for all other pathogens

For more information concerning any of these tests, please contact client care at clientcare@vet.k-state.edu or 866-512-5650.

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